Journal: Multiple sclerosis journal - experimental, translational and clinical

Article Title: Safety, tolerability and pharmacodynamics of a novel immunomodulator, MIS416, in patients with chronic progressive multiple sclerosis

doi: 10.1177/2055217315583385

Figure Lengend Snippet: Plasma from blood samples collected at 24 hours and seven days post MIS416 dosing from each patient was assayed for neopterin using flow cytometry bead-based ELISA technology. The data shown are the mean values (pg/mL) + SD ( n = 15). (DC Cohort; 500 µg/week.).

Article Snippet: All analytes were quantified using flow cytometry fluorescent bead-based multiplex assay (Becton Dickinson CBATM) according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients

doi: 10.1186/s12931-016-0436-2

Figure Lengend Snippet: Effects of dexamethasone, PIK-294 and tofacinib on TCR-induced pSTAT5 activation in lymphocytes. Isolated blood T-cells from asthma patients ( n = 5) and healthy subjects ( n = 6) and BAL cells ( n = 4 asthma plus n = 3 healthy) were treated with 1000nM dexamethasone, PIK-294 or tofacinib for 1 h before TCR-stimulation for 4 h. Levels of phosphorylated STAT5 in CD3 + cells were quantified by flow cytometry. Data is presented as mean % pSTAT5 positive CD3 cells +/− standard deviation. Drug effects were assessed by 1-way ANOVA with a Dunnett’s multiple comparison test against the stimulated no drug control: * p < 0.05

Article Snippet: Cytotoxic effects of the drugs were assessed in TCR-stimulated PBMCs by Pierce LDH assay (Life Technologies, Paisley, UK) and propidium iodide flow cytometry assay (BD Bioscience, Oxford, UK); Additional file : Figure S1.

Techniques: Activation Assay, Isolation, Flow Cytometry, Standard Deviation

Journal: Respiratory Research

Article Title: Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients

doi: 10.1186/s12931-016-0436-2

Figure Lengend Snippet: Expression of PI3Kδ in subepithelial and BAL T-cells from asthma patients and healthy subjects. PI3Kδ expression was examined in CD3 cells in subepithelial bronchial tissue and BAL by immunohistochemistry and flow cytometry, respectively. Representative images show PI3Kδ expression in bronchial tissue from healthy subjects ( a ) and asthma patients ( b ). Arrows and amplified images highlight example staining: Red for CD3 only; Blue for PI3Kδ only; Green for dual CD3 and PI3Kδ. Black scale bars represent 50 μm. Data is presented as ( c ) numbers of CD3 + PI3Kδ + cells per mm 2 subepithelium (Healthy n = 10; Asthma n = 9); ( d ) percentage of subepithelial CD3 cells expressing PI3Kδ (Healthy n = 8; Asthma n = 8); ( e ) percentage of BAL CD3 cells expressing PI3Kδ (Healthy n = 6; Asthma n = 9) and ( f ) PI3Kδ-dependent mean fluorescence intensity in dual labelled CD3 + PI3Kδ + BAL cells (Healthy n = 6; Asthma n = 9), with bar illustrating mean values. The percentage of subepithelial CD3 cells expressing PI3Kδ could not be calculated for n = 2 healthy and n = 1 asthma, due to lack of CD3 cells. Differences between healthy and asthma were assessed by T-test: * p < 0.05; ** p < 0.01

Article Snippet: Cytotoxic effects of the drugs were assessed in TCR-stimulated PBMCs by Pierce LDH assay (Life Technologies, Paisley, UK) and propidium iodide flow cytometry assay (BD Bioscience, Oxford, UK); Additional file : Figure S1.

Techniques: Expressing, Immunohistochemistry, Flow Cytometry, Amplification, Staining, Fluorescence